多光子顕微鏡 Bergamo® IIシリーズ

 

Feedback?

Questions?

Need a Quote?

ご質問やお見積りのご要望は
お気軽に当社までご連絡ください。

多光子顕微鏡 Bergamo^® IIシリーズ

当社では試料を顕微鏡に適合させるのではなく、顕微鏡を試料に適合させるべきであるという方針に基づき、様々な実験要件に対応可能なモジュール式の多光子イメージングプラットフォームを開発しました。当社の多光子イメージングシステムは、微小電極などを用いた従来の研究手法に比べて、高速、高深度、高強度でニューロン集団の同時読み出しと操作が可能です。 下のオプション一覧および右下の回転式Bergamo顕微鏡の5軸移動の動画でご紹介しているように、当社では回転式ボディを開発し、これにより顕微鏡が試料の周りを回転できるようになりました。当社では固定式ボディのタイプもご用意しています。このタイプではアーム下の奥行きが196.5 mmあり、対物レンズ周りに大きな作業スペースを3次元的に確保できます。

「ハイライト」、「特長」および「モジュール」タブでご紹介しているBergamo IIの特長には、操作性を損なうことなく最先端の性能を得ることに注力してきたことが反映されています。Bergamo顕微鏡の推奨用途についての詳しい説明は「用途」タブに記載されています。このプラットフォームは実験のニーズに応じて簡単に変更やアップグレードができます。対応可能なアドオンおよびアップグレードについては「レトロフィット」タブをご覧ください。

Bergamoイメージングシステムを使用することで、優れた試料イメージ(「イメージングギャラリ」タブ参照)を得ることができ、それらは数多くの出版物に掲載されています(「関連出版物」タブ参照)。 右下の「共同研究で生まれたイノベーション」の動画でご覧いただけるように、当社のライフサイエンス部門はUniversity College LondonのDr. Michael Hausserの研究グループと連携し、彼らのニーズに合ったカスタム仕様のBergamo顕微鏡システムを設計してきました。当社のBergamoイメージングシステムについてのご質問は、ボックス内のContact USボタンをクリックして当社までお問合せください。

ハイライト

• ベッセルビームを用いた高速ボリューム画像取得
• 3光子イメージング
• 空間光変調器(SLM)を用いた同時多点光刺激

ベッセルビームを用いた高速ボリューム画像取得
当社ではHoward Hughes Medical Institute(HHMI)とNa Ji教授(カリフォルニア大学バークレー校)の協力を得て、Bergamo^®多光子レーザ走査型顕微鏡用のベッセルビームモジュールをご提供しています。 神経細胞活動のin vivoボリュームイメージングを行うには、サブミクロンの空間分解能とミリ秒の時間分解能の両方が必要です。従来の方法では、回折限界のガウシアンビームを走査して3次元画像を作成しますが、ベッセルビームを用いた多光子イメージングでは、軸方向に細長い集光スポットを利用してボリューム画像を取得します。励起光の被写界深度が拡大されたことにより3次元の体積が2次元に投影され、2次元フレームレートは効率的に3次元ボリュームレートに変換されます。

Ji氏の先駆的な研究で実証されたように、このベッセルビームを用いた高速のイメージング技術はシナプス解像度を有し、Ca²⁺ダイナミクスや、マウスとフェレットの視覚皮質における樹状突起スパインの調整特性などの計測ができます。 下の画像ではこのベッセルビームを用いた多光子イメージング技術の能力を示しています。Thy1-GFP-Mマウスの脳切片の300 x 300 μmの範囲を、それぞれベッセルビーム(左)およびガウシアンビーム(右)で走査した画像を比較しています。ガウシアンビームを集光して取得された45枚の光学的なスライス画像を垂直に積み重ねるとボリュームイメージが得られますが、長さ45 μmの集光スポットを有するベッセルビームを使用すると、1回走査するだけで同じ構造的な特徴を見ることができます。これはボリュームイメージングの速度が大幅に向上することを示しており、この手法はin-vivoでまばらにラベル付けされた試料を調べるのに適しています。

お持ちのBergamo顕微鏡にベッセルビームによるイメージング機能の追加を希望される場合は、当社までご連絡ください。ご提供可能なアップグレードとアドオンについては、「レトロフィット」のタブをご覧ください。

出典: Lu R, Sun W, Liang Y, Kerlin A, Bierfeld J, Seelig JD, Wilson DE, Scholl B, Mohar B, Tanimoto M, Koyama M, Fitzpatrick D, Orger MB, and Ji N. "Video-rate volumetric functional imaging of the brain at synaptic resolution." Nature Neuroscience. 2017 Feb 27; 20: 620-628.

Click to Enlarge

Click to Enlarge

1回のベッセルビームによる走査画像(左)は、ガウシアンビームによる45枚の光学画像の断片を積み重ねて得られた体積走査画像(右)と同じ構造情報を捉えており、これは全走査時間を45分の1に短縮できることを示しています。これらの画像は脳切片の300 μm x 300 μmの範囲を走査したものです。ガウシアンビームによる画像を積み重ねた画像の深さはスケールバーで示されています。試料ご提供：Qinrong Zhang, PhD and Matthew Jacobs; the Ji Lab, Department of Physics, University of California, Berkeley.

Click to Enlarge
Thy1-YFPマウス(オス、21週)の画像。1300 nm、繰り返し周波数326 kHz、パルス幅約60 fsで取得。観察窓の中心は、体性感覚皮質上部、ブレグマから横方向に2.5 mm、後方に2 mmの位置にあります(皮質表面でのレーザーパワーは1.1 mW)。画像ご提供:Chris Xu Group, Cornell University.

3光子イメージング
当社では、Bergamo多光子顕微鏡に3光子イメージング技術を導入できるように、800～1800 nmの波長範囲に対応する走査光路用の光学素子を開発しました。3光子励起は深部組織イメージングに適していますが、一般には波長1300 nmまたは1700 nm付近の高パルスエネルギーの励起光源が必要です。2光子イメージングと比較して、3光子イメージングでは組織による散乱が少なく、また焦点の合わないバックグラウンド光が抑制されるので、信号対バックグラウンドの比が改善されます。

下の写真のように、3光子イメージングが可能なシステムの構成要素として、2光子と3光子の同時イメージングをサポートするダイクロイックミラーや、広帯域でのサンプリングができる繰り返し周波数の低いレーザをサポートするエレクトロニクスを含めることができます。ThorImage^®LSソフトウェアでは、3光子検出のための重要な機能を強化しています。例えば、3光子信号の検出を励起パルスと同期させ、最良の信号対雑音比が得られるように位相遅延を制御することができます。詳細については「ThorImageLS」タブをご参照ください。

お持ちのBergamo顕微鏡に3光子イメージング機能の追加を希望される場合は、当社までご連絡ください。ご提供可能なアップグレードとアドオンについては、「レトロフィット」タブをご覧ください。

出典:  Wang T and Xu C. "Three-photon neuronal imaging in deep mouse brain." Optica. 2020; 7 (8): 947-960.

Click to Enlarge
2光子および3光子イメージングの構成。

空間光変調器(SLM)を用いた同時多点光刺激
当社の空間光変調器(SLM)を用いると、ホログラフィックパターンにより試料内の複数の位置の光刺激を同時に行うことができます。この空間光変調器はフェムト秒パルスレーザによる2光子励起用に設計されており、光刺激用レーザのビームプロファイル全体に渡って位相を操作し、ユーザが決定した数百の焦点を生成することができます。

下の図では、空間光変調器を使用した2光子刺激の利点を、通常の2光子刺激や単一光子による光刺激と比較して示しています。単一光子による光刺激では、1つの細胞だけをターゲットにすることができず、ターゲットとする細胞に近接する細胞も刺激してしまいます。この問題は、1つの細胞をターゲットにできる分解能を有する2光子刺激を利用することで解決します。しかし、その場合でも同時にターゲットにできる細胞は1つに制限されます。2光子刺激と空間光変調器を組み合わせると、多数の焦点を生成して複数の細胞を同時に刺激することができるため、その制限を克服することができます。刺激用ビームの形状は光刺激の効果が向上するように成形することができます。これは、単一視野内の異なる深度にある神経集団を活性化させるために重要な機能です。 空間光変調器の位相マスクのパターンは素早く切り替えることができ、複数の独立した焦点を任意のシーケンスで個々のターゲットに向けることができます。校正プロセス、ホログラムの生成、外部ハードウェアとの同期などは、すべてThorImage^®ソフトウェアで管理され、シームレスな制御が可能です。 詳細については「ThorImageLS」タブをご覧ください。

お持ちのBergamo顕微鏡に空間光変調器(SLM)によるイメージング機能の追加を希望される場合は、当社までご連絡ください。ご提供可能なアップグレードとアドオンについては、「レトロフィット」タブをご覧ください。

2光子刺激+空間光変調器

• 複数の焦点を生成
• 複数のターゲット細胞を同時に刺激

Click to Enlarge

2光子刺激

• 1つの細胞をターゲットにできる分解能
• ターゲットとする細胞のみを刺激

Click to Enlarge

単一光子による刺激

• 3次元的に神経を刺激するには深さの調整能力が不足
• ターゲット細胞とそれに近接する細胞を刺激

Click to Enlarge

特長　

Bergamo^®IIシリーズ顕微鏡の下記の多くの機能は、既存の当社顕微鏡システムにも追加できます。詳細については「レトロフィット」のタブをご参照ください。　

Bergamo^® IIシリーズモジュール

当社のBergamo^® II顕微鏡は、実験の要件に合わせ構成をカスタマイズ可能なモジュール式システムです。

Click to Enlarge
図2.ガウシアンビーム

Click to Enlarge
図1. ベッセルビーム

ベッセルビームを使用したボリュームイメージング手法　

こちらは、ベッセルビームを使用した当社の新しい超高速イメージング手法です。神経回路や相互作用のin vivoボリューム機能イメージングをビデオレートで実現します。この独特なビームは、非回折で自己修復性をもつため、強く集光され、組織を透過しても再成形されます。この技術は、当社のBergamo II多光子顕微鏡と2光子メゾスコープに採用されます。 

右はベッセルビームとガウシアンビームの画像です。画像でご覧いただけるように、ガウシアンビームの焦点は1点で、中心から広がるにつれ集光は弱まります。一方ベッセルビームは焦点を保つ円環ビームとなります。　

こちらの技術に関するプレスリリースはこちらからご覧いただけます。

Click to Enlarge
回転式Bergamo IIシリーズシステムには多関節式ペリスコープが付いています。このペリスコープの設計により、走査システム全体を試料に対して傾けられるなど、柔軟性が強化されています。

Click to Enlarge
正立顕微鏡型Bergamo IIシリーズシステムには、光学性能を損なうことなく顕微鏡のXYZ各軸の全移動範囲が使用可能となるペリスコープが付いています。

Click to Enlarge
吸収フィルタとダイクロイックキューブは、PMT検出モジュールの前にある磁石で密閉されたドアの向こう側で保持されています。

構成例の集光素子^a

同時多点光刺激(回転式)	反射方向 10°
2光子または3光子イメージング(回転式)	反射方向: 14°
ランダムアクセス走査(回転式)	反射方向: 14°
in vivo 2光子イメージング(回転式)	反射方向: 14°
デュアル光路ランダムアクセス走査(XYZ)	反射方向: 14° 透過方向: 13°
デュアル走査共焦点イメージング(XYZ)	反射方向: 14°
シンプルなイメージング(XYZ)	反射方向: 8° 透過方向: 13°
動画ならびに高速イメージング(Zのみ)	反射方向: 8°
シンプルなイメージング(Zのみ)	反射方向: 8°

Click to Enlarge

Click to Enlarge

Click to Enlarge

Click to Enlarge
sCMOSカメラZeluxとQuantulux

Click to Enlarge
対物レンズの下にある透過モジュール、電動式コンデンサ、そして高剛性スタンドサンプルホルダ

Thorlabs' Bergamo^® II Series Multiphoton Microscopy Platform is a powerful tool adapted to meet experimental needs across a wide range of research fields. Click on the images below to explore how a Bergamo can and has been utilized for each application.

Structural Neurobiology

Neurological Disorders

Neural Development and Plasticity

Neurogenetics

Functional and Molecular Imaging

Synapses and Circuits

Ion Channels, Transporters, and Neurotransmitter Reporters

Cell Biology of Neurons, Muscles, and Glia

Drug Discovery

Close

Click for Full Size Image

Top: Astrocytes are labelled with SR101 (red). Arrows point to astrocytes that had Ca²⁺ elevations during tDCS. The numbers correspond to the cells and neurogliopil regions plotted in the graphs below. Bottom: Fluorescent intensity (ΔF/F) traces of astrocytes (orange), neurons (green) and neurogliopil (brown). Figure Courtesy of Monai H. et al. (See Below)

Application Summary

The study of cell biology, muscles, and glia often involves imaging in vitro or in vivo samples tagged with multiple fluorophores. Through multiphoton imaging with these fluorescent markers, researchers are able to observe gene expression related to neural function in different areas of the brain. We recommend two of our configurations for this application, see the table below. Each configuration has a small footprint and a large throat depth to provide ample room for numerous sample mounting options, including in vivo imaging of mammalian brains via transcranial windows.

Body	Upright XYZ	Upright Z-Axis
Function	Simple XYZ Imaging	Video and High-Speed Imaging

Publications

Rose T, Jaepel J, Hübener M, and Bonhoeffer T. "Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex." Science. 2016 Jun 10; 352 (6291): 1319-1322.

Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, Mikoshiba K, Itohara S, Nakai J, Iwai Y, and Hirase H. "Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain." Nature Communications. 2016 March 22; 7: 11100.

Lu W, Xie Z, Tang Y, Bai L, Yao Y, Fu C, and Ma G. "Photoluminescent mesoporous silicon nanoparticles with siccr2 improve the effects of mesenchymal stromal cell transplantation after acute myocardial infarction." Theranostics. 2015 Jun 25; 5 (10): 1068-1082.

Qin X, Qiu C, and Zhao L. "Maslinic acid protects vascular smooth muscle cells from oxidative stress through Akt/Nrf₂/HO-₁ pathway." Molecular and Cellular Biochemistry. 2014 Feb 20; 390 (1-2): 61-67.

Close

Click for Full Size Image

Artistic Rendering of DNA

Application Summary

Drug discovery research is rapidly expanding and often requires a wide variety of experimental setups and imaging techniques. Two-photon imaging is frequently used to measure the characteristics of drug applications, including depth of drug penetration and the area of its spread throughout the cortex. We offer two configurations that are suitable for this application, see the table below. These configurations are are well-balanced for both in vitro and fixed stage in vivo microscopy research. The modularity of the Bergamo systems' removable trans-illumination module provides versatility in regard to experimental techniques, imaging modalities, and sample subjects.

Body	Upright XYZ
Function	Simple XYZ Imaging	Bessel Beam

Publications

Strobl MJ, Freeman D, Patel J, Poulsen R, Wendler CC, Rivkees SA, and Coleman E. " Opposing effects of maternal hypo- and hyperthyroidism on the stability of thalamocortical synapses in the visual cortex of adult offspring." Cerebral Cortex. 2017 May 1; 27 (5): 3015-3027.

Scattolini V, Luni C, Zambon A, Galvanin S, Gagliano O, Ciubotaru CD, Avogaro A, Mammano F, Elvassore N, and Fadini GP. "Simvastatin rapidly and reversibly inhibits insulin secretion in intact single-islet cultures." Diabetes Therapy. 2016 Nov 9; 7 (4): 679-693.

Close

Click for Full Size Image

Two-Photon Image of Neurons Expressing Thy1-YFP in a Cleared Region of the Hippocampus, Courtesy of the 2017 Imaging Structure and Function in the Nervous System Course at Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

Application Summary

Scientists interested in this application focus on tracing neuronal pathways and researching dendritic spine plasticity. Imaging systems used in this type of research are capable of two-photon calcium imaging and/or confocal fluorescence imaging. High-resolution and high-sensitivity are crucial features for these systems. We offer three multiphoton configurations that are ideal for this application (see the table below). Each configuration may be used in photon-limited environments as they feature sensitive detection modules, in addition to our 10° or 14° full field-of-view collection optics with ultrasensitive GaAsP PMTs. Our Dual-Path with Confocal Imaging configuration allows for a range of experimental conditions to be observed using any combination of multiphoton, confocal, and epi-fluorescence imaging, along with photoactivation. Alternatively, the Video and High-Speed Imaging and Simple Z-Axis Imaging configurations provide a small footprint and large throat depth for in vivo two-photon imaging.

Body	Upright XYZ	Upright Z-Axis
Function	Dual Path with Confocal Imaging	Video and High-Speed Imaging	Simple Z-Axis Imaging

Publications

Gillet SN, Kato HK, Justen MA, Lai M, and Isaacson JS. "Fear learning regulates cortical sensory representations by suppressing habituation." Frontiers in Neural Circuits. 2018 Jan 10; 11: 112.

Micu I, Brideau C, Lu L, and Stys PK. "Effects of laser polarization on responses of the fluorescent Ca²⁺ indicator X-Rhod-1 in neurons and myelin." Neurophotonics. 2017 Jun 5; 4 (2): 025002.

Chen SX, Kim AN, Peters AJ, and Komiyama T. "Subtype-specific plasticity of inhibitory circuits in motor cortex during motor learning." Nature Neuroscience. 2015 Jun 22; 18: 1109-1115.

Peters AJ, Chen SX, and Komiyama T. "Emergence of reproducible spatiotemporal activity during motor learning." Nature. 2014 Jun 12; 510: 263-267.

Close

Click for Full Size Image

Stitched Confocal Fluorescence Image of Rat Retina Stained with DAPI, Alexa Fluor^® 555 and Alexa Fluor^® 633, Courtesy of Dr. Jennifer Kielczewski, National Eye Institute, National Institutes of Health, Bethesda, MD

Application Summary

Researching neurological disorders involves measuring neural function using two-photon calcium imaging. This research requires fast image acquisition and photostimulation. We recommend three of our multiphoton microscope configurations for this application (see the table below). Each configuration offers a large working space and rotating microscope body, making it ideal for in vivo animal studies. Our Multi-Target Photoactivation configuration features a spatial light modulator (SLM), which allows the activation of groups of neurons at varying depths within a single field of view. For increased penetration of samples with scattering tissues, the three-photon capability of our Two- and Three-Photon Imaging configuration is recommended. Our Dual-Modality and In Vivo Imaging configuration uses a resonant-galvo-galvo scanner to take high-resolution images. This scanner provides all the speed of a resonant-galvo scanner, while enabling user-defined photoactivation regions.

Body	Rotating
Function	Multi-Target Photoactivation	Two- and Three-Photon Imaging	Dual-Modality and In Vivo Imaging

Publications

Murphy SC, Palmer LM, Nyffeler T, Müri RM, and Larkum ME. "Transcranial magnetic stimulation (TMS) inhibits cortical dendrites." eLife. 2016 Mar 18; 5: e13598.

Lalchandani RR, van der Goes MS, Partridge JG, and Vicini S. "Dopamine D₂ receptors regulate collateral inhibition between striatal medium spiny neurons." The Journal of Neuroscience. 2013 Aug 28; 33 (35): 14075-14086.

Click to Enlarge
回転式Bergamo IIシステム用の透過光路モジュール

既存の多光子イメージングシステム向けの追加オプション

• 既存の顕微鏡基盤ならびに部品の機能アップグレード　
• 部品の追加による機能向上　

当社のモジュール設計の顕微鏡は、実験ニーズに応じて常に進化させることができます。旧モデルの顕微鏡や多光子顕微鏡製品も、必要に応じ、基盤のアップグレードや既存部品に機能を追加できる柔軟性が備わっています。当社の多光子システムのラインナップの追加オプションについては下表をご覧いただき、アップグレードもしくはアドオンの詳細については当社までご連絡ください。　

なお、アップグレードやアドオンの設置には担当者が訪問させていただく場合があることをご了承ください。

Images Taken Using Bergamo^® Systems

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Click for Details

Selected Publications Using Thorlabs' Imaging Systems

2023

Tsuji, M., Nishizuka, Y. & Emoto, K. (2023). Threat gates visual aversion via theta activity in Tachykinergic neurons. Nature Communications, 14(1), 3987. 

Villanueva, C. B., Stephensen, H. J. T., Mokso, R., Benraiss, A., Sporring, J., Goldman, S.A. (2023). Astrocytic engagement of the corticostriatal synaptic cleft is disrupted in a mouse model of Huntington's disease. Proceedings of the National Academy of Sciences, 120(24), e2210719120.

Holstein-Rønsbo, S., Gan, Y., Giannetto, M.J. et al. (2023). Glymphatic influx and clearance are accelerated by neurovascular coupling. Nature Neuroscience, 26, 1042–1053.

Serradj, N., Marino, F., Moreno-López, Y. et al. (2023). Task-specific modulation of corticospinal neuron activity during motor learning in mice. Nature Communications, 14(1), 2708.

Inayat, S., McAllister, B. B., Whishaw, I. Q., & Mohajerani, M. H. (2023). Hippocampal conjunctive and complementary CA1 populations relate sensory events to movement. Iscience, 26(4), 106481.

Untiet, V., Beinlich, F. R., Kusk, P., Kang, N., Ladrón-de-Guevara, A., Song, W., ... & Nedergaard, M. (2023). Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice. Nature Communications, 14(1), 1871.

Anthoney, N., Tainton-Heap, L., Luong, H., Notaras, E., Zhao, Q., Perry, T., ... & van Swinderen, B. (2023). Experimentally induced active and quiet sleep engage non-overlapping transcriptomes in Drosophila. bioRxiv, 535331.

Boster, K. A., Cai, S., Ladrón-de-Guevara, A., Sun, J., Zheng, X., Du, T., ... & Kelley, D. H. (2023). Artificial intelligence velocimetry reveals in vivo flow rates, pressure gradients, and shear stresses in murine perivascular flows. Proceedings of the National Academy of Sciences, 120(14), e2217744120.

Scarpetta, V., Bodaleo, F., Salio, C., Agarwal, A., Sassoè-Pognetto, M., & Patrizi, A. (2023). Morphological and mitochondrial changes in murine choroid plexus epithelial cells during healthy aging. Fluids and Barriers of the CNS, 20(1), 1-16.

McDermott, K. D., Frechou, M. A., Jordan, J. T., Martin, S. S., & Gonçalves, J. T. (2023). Delayed formation of neural representations of space in aged mice. bioRxiv, 531021.

Matthews, M. D., Cook, E., Naguib, ,N., Wiesner, U. B., & Lewis, K. J. (2023). Intravital imaging of osteocyte integrin dynamics with locally injectable fluorescent nanoparticles. Bone, 174, 116830.

Tort-Colet, N., Resta, F., Montagni, E., Pavone, F., Allegra Mascaro, A. L., & Destexhe, A. (2023). Assessing brain state and anesthesia level with two-photon calcium signals. Scientific Reports, 13(1), 3183.

Esteves, I. M., Chang, H., Neumann, A. R., & McNaughton, B. L. (2023). Consolidation of cellular memory representations in superficial neocortex. Iscience, 26(2), 105970.

Quezada, A., Ward, C., Bader, E. R., Zolotavin, P., Altun, E., Hong, S., ... & Hébert, J. M. (2023). An in vivo platform for rebuilding functional neocortical tissue. Bioengineering, 10(2), 263.

Yang, J. Y., O’Connell, T. F., Hsu, W. M. M., Bauer, M. S., Dylla, K. V., Sharpee, T. O., & Hong, E. J. (2023). Restructuring of olfactory representations in the fly brain around odor relationships in natural sources. bioRxiv, 528627.

Carlton, A. J., Jeng, J. Y., Grandi, F. C., De Faveri, F., Ceriani, F., De Tomasi, L., ... & Marcotti, W. (2023). A critical period of prehearing spontaneous Ca2+ spiking is required for hair-bundle maintenance in inner hair cells. The EMBO Journal, 42(4), e112118.

Hamling, K. R., Zhu, Y., Auer, F., & Schoppik, D. (2023). Tilt In Place Microscopy (TIPM): a simple, low-cost solution to image neural responses to body rotations. Journal of Neuroscience, 43(6), 936-948.

Vestergaard, M., Carta, M., Güney, G., & Poulet, J. F. A. (2023). The cellular coding of temperature in the mammalian cortex. Nature, 614(7949), 725-731.

Contreras-López, R., Alatriste-León, H., Díaz-Hernández, E., Ramírez-Jarquín, J. O., & Tecuapetla, F. (2023). The deep cerebellar nuclei to striatum disynaptic connection contributes to skilled forelimb movement. Cell Reports, 42(1), 112000.

Mabuchi, Y., Cui, X., Xie, L., Kim, H., Jiang, T., & Yapici, N. (2023). GABA-mediated inhibition in visual feedback neurons fine-tunes Drosophila male courtship. bioRxiv, 525544.

Das, A., Margevicius, D., Borovicka, J., Icardi, J., Patel, D., Paquet, M. E., & Dana, H. (2023). Enhanced detection sensitivity of neuronal activity patterns using CaMPARI1 vs. CaMPARI2. Frontiers in Neuroscience, 16, 2291.

Lee, S., Lee, K., Choi, M., & Park, J. (2023). Implantable acousto-optic window for monitoring ultrasound-mediated neuromodulation in vivo. Neurophotonics, 9(3), 032203.

Zhuo, G. Y., Chen, M. C., Lin, T. Y., Lin, S. T., Chen, D. T. L., & Lee, C. W. S. (2023). Opioid-Modulated Receptor Localization and Erk1/2 Phosphorylation in Cells Coexpressing μ-Opioid and Nociceptin Receptors. International Journal of Molecular Sciences, 24(2), 1048.

Diaz-Cuadros, M., Miettinen, T. P., Skinner, O. S., Sheedy, D., Díaz-García, C. M., Gapon, S., ... & Pourquié, O. (2023). Metabolic regulation of species-specific developmental rates. Nature, 613(7944), 550-557.

Evrard, M., Mansuryan, T., Couderc, V., Désévédavy, F., Strutynski, C., Dussauze, M., ... & Smektala, F. (2023). Highly Nonlinear Multimode Tellurite Fibers: From Glass Synthesis to Practical Applications in Multiphoton Imaging. Advanced Photonics Research, 4(1), 2200213.

2022

Graham, R. T., Parrish, R. R., Alberio, L., Johnson, E. L., Owens, L., & Trevelyan, A. J. (2022). Optogenetic stimulation reveals a latent tipping point in cortical networks during ictogenesis. Brain, awac487.

Shiozaki, H. M., Wang, K., Lillvis, J. L., Xu, M., Dickson, B. J., & Stern, D. L. (2022). Neural coding of distinct motor patterns during Drosophila courtship song. bioRxiv, 520499.

Fisher, Y. E., Marquis, M., D’Alessandro, I., & Wilson, R. I. (2022). Dopamine promotes head direction plasticity during orienting movements. Nature, 612(7939), 316-322.

Wang, X., Delle, C., Asiminas, A., Akther, S., Vittani, M., Brxxxgger, P., ... & Hirase, H. (2022). Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. Cell Reports Methods, 2(10), 100302.

Lu, Y., Wei, X., Li, W., Wu, X., Chen, C., Li, G., ... & Gan, W. B. (2022). Large-volume and deep brain imaging in rabbits and monkeys using COMPACT two-photon microscopy. Scientific Reports, 12(1), 17736.

Huang, J. S., Kunkhyen, T., Rangel, A. N., Brechbill, T. R., Gregory, J. D., Winson-Bushby, E. D., ... & Cheetham, C. E. (2022). Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb. Nature Communications, 13(1), 6194.

Govindaraju, I., Muraleedharan, M., Maidin, S., Chakraborty, I., Zhuo, G. Y., Mahato, K. K., & Mazumder, N. (2022). Investigation of the effect of gamma irradiation on the morphological structures of starch using advanced microscopic techniques. In Frontiers in Optics, JTu5A-74.

Hwang, F. J., Roth, R. H., Wu, Y. W., Sun, Y., Kwon, D. K., Liu, Y., & Ding, J. B. (2022). Motor learning selectively strengthens cortical and striatal synapses of motor engram neurons. Neuron, 110(17), 2790-2801.

Yue, Y., Ash, R. T., Boyle, N., Kinter, A., Li, Y., Zeng, C., & Lu, H. (2022). MeCP2 deficiency impairs motor cortical circuit flexibility associated with motor learning. Molecular Brain, 15(1), 1-12.

Liu, Z., Lu, X., Villette, V., Gou, Y., Colbert, K. L., Lai, S., Guan, S., ... & St-Pierre, F. (2022). Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell, 185(18), 3408-3425.

Aragon, M. J., Mok, A. T., Shea, J., Wang, M., Kim, H., Barkdull, N., ... & Yapici, N. (2022). Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle. Elife, 11, e69094.

Matheson, A. M., Lanz, A. J., Medina, A. M., Licata, A. M., Currier, T. A., Syed, M. H., & Nagel, K. I. (2022). A neural circuit for wind-guided olfactory navigation. Nature Communications, 13(1), 4613.

Chornyy, S., Borovicka, J. A., Patel, D., Shin, M. K., Vázquez-Rosa, E., Miller, E., ... & Dana, H. (2022). Longitudinal in vivo monitoring of axonal degeneration after brain injury. Cell Reports Methods, 3(5), 100481.

Sheng, W., Zhao, X., Huang, X., & Yang, Y. (2022). Real-time image processing toolbox for all-optical closed-loop control of neuronal activities. Frontiers in Cellular Neuroscience, 16, 917713.

Tadres, D., Shiozaki, H. M., Tastekin, I., Stern, D. L., & Louis, M. (2022). An essential experimental control for functional connectivity mapping with optogenetics. bioRxiv, 493610.

Koveal, D., Rosen, P. C., Meyer, D. J., Díaz-García, C. M., Wang, Y., Cai, L. H., ... & Yellen, G. (2022). A high-throughput multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors. Nature Communications, 13(1), 2919.

Fukuda, M., Matsumura, T., Suda, T., & Hirase, H. (2022). Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain. Neurophotonics, 9(2), 021910-021910.

Jackson, J. G., Krizman, E., Takano, H., Lee, M., Choi, G. H., Putt, M. E., & Robinson, M. B. (2022). Activation of Glutamate Transport Increases Arteriole Diameter in vivo: Implications for Neurovascular Coupling. Frontiers in Cellular Neuroscience, 16, 831061.

Du, T., Mestre, H., Kress, B. T., Liu, G., Sweeney, A. M., Samson, A. J., ... & Nedergaard, M. (2022). Cerebrospinal fluid is a significant fluid source for anoxic cerebral oedema. Brain, 145(2), 787-797.

Govindaraju, I., Zhuo, G. Y., Chakraborty, I., Melanthota, S. K., Mal, S. S., Sarmah, B., ... & Mazumder, N. (2022). Investigation of structural and physico-chemical properties of rice starch with varied amylose content: A combined microscopy, spectroscopy, and thermal study. Food Hydrocolloids, 122, 107093.

Choe, K., Hontani, Y., Wang, T., Hebert, E., Ouzounov, D. G., Lai, K., ... & Xu, C. (2022). Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes. Nature Immunology, 23(2), 330-340.

Spivak, Y. M., & Lemeshko, P. S. (2022). Multiphoton microscopy of mesoporous silicon. In Journal of Physics: Conference Series. 2227(1), 012013.

2021

Maset, A., Albanesi, M., di Soccio, A., Canova, M., Dal Maschio, M., & Lodovichi, C. (2021). Aberrant Patterns of Sensory-Evoked Activity in the Olfactory Bulb of LRRK2 Knockout Mice. Cells, 10(11), 3212.

Schmidt, E. R., Zhao, H. T., Park, J. M., Dipoppa, M., Monsalve-Mercado, M. M., Dahan, J. B., ... & Polleux, F. (2021). A human-specific modifier of cortical connectivity and circuit function. Nature, 599(7886), 640-644.

Hermans, L., Kaynak, M., Braun, J., Lobato Ríos, V., Chen, C. L., Günel, S., ... & Ramdya, P. (2021). Long-term imaging of the ventral nerve cord in behaving adult Drosophila. bioRxiv, 463778.

Carlsen, E. M. M., Falk, S., Skupio, U., Robin, L., Pagano Zottola, A. C., Marsicano, G., & Perrier, J. F. (2021). Spinal astroglial cannabinoid receptors control pathological tremor. Nature Neuroscience, 24(5), 658-666.

Moussa, N. O., Mansuryan, T., Hage, C. H., Fabert, M., Krupa, K., Tonello, A., ... & Couderc, V. (2021). Spatiotemporal beam self-cleaning for high-resolution nonlinear fluorescence imaging with multimode fiber. Scientific Reports, 11(1), 18240.

Eleftheriou, C. G., Corona, C., Khattak, S., Alam, N. M., Ivanova, E., Bianchimano, P., ... & Sagdullaev, B. T. (2021). Retinoschisin deficiency induces persistent aberrant waves of activity affecting neuroglial signaling in the retina. Journal of Neuroscience, 42(36), 6983-7000.

Han, J., Kim, S., Choi, P., Lee, S., Jo, Y., Kim, E., & Choi, M. (2021). Robust functional imaging of taste sensation with a Bessel beam. Biomedical Optics Express, 12(9), 5855-5864.

Favuzzi, E., Huang, S., Saldi, G. A., Binan, L., Ibrahim, L. A., Fernández-Otero, M., ... & Fishell, G. (2021). GABA-receptive microglia selectively sculpt developing inhibitory circuits. Cell, 184(15), 4048-4063.

Chornyy, S., Das, A., Borovicka, J. A., Patel, D., Chan, H. H., Hermann, J. K., ... & Dana, H. (2021). Cellular-resolution monitoring of ischemic stroke pathologies in the rat cortex. Biomedical Optics Express, 12(8), 4901-4919.

Malina, K. C. K., Tsivourakis, E., Kushinsky, D., Apelblat, D., Shtiglitz, S., Zohar, E., ... & Spiegel, I. (2021). NDNF interneurons in layer 1 gain-modulate whole cortical columns according to an animal’s behavioral state. Neuron, 109(13), 2150-2164.

Bruzzone, M., Chiarello, E., Albanesi, M., Miletto Petrazzini, M. E., Megighian, A., Lodovichi, C., & Dal Maschio, M. (2021). Whole brain functional recordings at cellular resolution in zebrafish larvae with 3D scanning multiphoton microscopy. Scientific Reports, 11(1), 11048.

Mridha, Z., de Gee, J. W., Shi, Y., Alkashgari, R., Williams, J., Suminski, A., ... & McGinley, M. J. (2021). Graded recruitment of pupil-linked neuromodulation by parametric stimulation of the vagus nerve. Nature Communications, 12(1), 1539.

Ivanova, E., Corona, C., Eleftheriou, C. G., Bianchimano, P., & Sagdullaev, B. T. (2021). Retina-specific targeting of pericytes reveals structural diversity and enables control of capillary blood flow. Journal of Comparative Neurology, 529(6), 1121-1134.

Hung, C. W., Mazumder, N., Lin, D. J., Chen, W. L., Lin, S. T., Chan, M. C., & Zhuo, G. Y. (2021). Label-free characterization of collagen crosslinking in bone-engineered materials using nonlinear optical microscopy. Microscopy and Microanalysis, 27(3), 587-597.

Hontani, Y., Xia, F., & Xu, C. (2021). Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain. Science Advances, 7(12), eabf3531.

Resnik, J., & Polley, D. B. (2021). Cochlear neural degeneration disrupts hearing in background noise by increasing auditory cortex internal noise. Neuron, 109(6), 984-996.

Tainton-Heap, L. A., Kirszenblat, L. C., Notaras, E. T., Grabowska, M. J., Jeans, R., Feng, K., ... & van Swinderen, B. (2021). A paradoxical kind of sleep in Drosophila melanogaster. Current Biology, 31(3), 578-590.

Hortholary, T., Carrion, C., Chouzenoux, E., Pesquet, J. C., & Lefort, C. (2021). Multiplex-multiphoton microscopy and computational strategy for biomedical imaging. Microscopy Research and Technique, 84(7), 1553-1562.

Clayton, K. K., Williamson, R. S., Hancock, K. E., Tasaka, G. I., Mizrahi, A., Hackett, T. A., & Polley, D. B. (2021). Auditory corticothalamic neurons are recruited by motor preparatory inputs. Current Biology, 31(2), 310-321.

Esteves, I. M., Chang, H., Neumann, A. R., Sun, J., Mohajerani, M. H., & McNaughton, B. L. (2021). Spatial information encoding across multiple neocortical regions depends on an intact hippocampus. Journal of Neuroscience, 41(2), 307-319.

Schumacher, J. W., McCann, M. K., Maximov, K. J., & Fitzpatrick, D. (2021). Selective enhancement of neural coding in V1 underlies fine-discrimination learning in tree shrew. Current Biology, 32(15), 3245-3260.

2020

Keller, A. J., Dipoppa, M., Roth, M. M., Caudill, M. S., Ingrosso, A., Miller, K. D., & Scanziani, M. (2020). A disinhibitory circuit for contextual modulation in primary visual cortex. Neuron, 108(6), 1181-1193.

Lu, J., Behbahani, A. H., Hamburg, L., Westeinde, E. A., Dawson, P. M., Lyu, C., ... & Wilson, R. I. (2020). Transforming representations of movement from body-to world-centric space. Nature, 601(7891), 98-104.

Scholl, B., Thomas, C. I., Ryan, M. A., Kamasawa, N., & Fitzpatrick, D. (2020). Cortical response selectivity derives from strength in numbers of synapses. Nature, 590(7844), 111-114.

Robinson, N. T., Descamps, L. A., Russell, L. E., Buchholz, M. O., Bicknell, B. A., Antonov, G. K., ... & Häusser, M. (2020). Targeted activation of hippocampal place cells drives memory-guided spatial behavior. Cell, 183(6), 1586-1599.

Fan, J. L., Rivera, J. A., Sun, W., Peterson, J., Haeberle, H., Rubin, S., & Ji, N. (2020). High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. Nature Communications, 11(1), 6020.

Okubo, T. S., Patella, P., D’Alessandro, I., & Wilson, R. I. (2020). A neural network for wind-guided compass navigation. Neuron, 107(5), 924-940.

Mazzarda, F., D'Elia, A., Massari, R., De Ninno, A., Bertani, F. R., Businaro, L., ... & Mammano, F. (2020). Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca 2+ signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea. Lab on a Chip, 20(16), 3011-3023.

Bowen, Z., Winkowski, D. E., & Kanold, P. O. (2020). Functional organization of mouse primary auditory cortex in adult C57BL/6 and F1 (CBAxC57) mice. Scientific Reports, 10(1), 10905.

Feng, K., Sen, R., Minegishi, R., Dübbert, M., Bockemühl, T., Büschges, A., & Dickson, B. J. (2020). Distributed control of motor circuits for backward walking in Drosophila. Nature Communications, 11(1), 6166.

Keller, A. J., Roth, M. M., & Scanziani, M. (2020). Feedback generates a second receptive field in neurons of the visual cortex. Nature, 582(7813), 545-549.

Eschbach, C., Fushiki, A., Winding, M., Afonso, B., Andrade, I. V., Cocanougher, B. T., ... & Zlatic, M. (2020). Circuits for integrating learnt and innate valences in the fly brain. bioRxiv, 058339.

Montgomery, M. K., Kim, S. H., Dovas, A., Zhao, H. T., Goldberg, A. R., Xu, W., ... & Hillman, E. M. (2020). Glioma-induced alterations in neuronal activity and neurovascular coupling during disease progression. Cell Reports, 31(2), 107500.

Shiozaki, H. M., Ohta, K., & Kazama, H. (2020). A multi-regional network encoding heading and steering maneuvers in Drosophila. Neuron, 106(1), 126-141.

Mestre, H., Du, T., Sweeney, A. M., Liu, G., Samson, A. J., Peng, W., ... & Nedergaard, M. (2020). Cerebrospinal fluid influx drives acute ischemic tissue swelling. Science, 367(6483), eaax7171.

Jeng, J. Y., Ceriani, F., Hendry, A., Johnson, S. L., Yen, P., Simmons, D. D., ... & Marcotti, W. (2020). Hair cell maturation is differentially regulated along the tonotopic axis of the mammalian cochlea. The Journal of Physiology, 598(1), 151-170.

Kovacs-Oller, T., Ivanova, E., Bianchimano, P., & Sagdullaev, B. T. (2020). The pericyte connectome: spatial precision of neurovascular coupling is driven by selective connectivity maps of pericytes and endothelial cells and is disrupted in diabetes. Cell Discovery, 6(1), 39.

Oe, Y., Wang, X., Patriarchi, T., Konno, A., Ozawa, K., Yahagi, K., ... & Hirase, H. (2020). Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance. Nature Communications, 11(1), 471.

2019

Romero, S., Hight, A. E., Clayton, K. K., Resnik, J., Williamson, R. S., Hancock, K. E., & Polley, D. B. (2019). Cellular and widefield imaging of sound frequency organization in primary and higher order fields of the mouse auditory cortex. Cerebral Cortex, 30(3), 1603-1622.

Nardin, C., Peres, C., Mazzarda, F., Ziraldo, G., Salvatore, A. M., & Mammano, F. (2019). Photosensitizer activation drives apoptosis by interorganellar Ca2+ transfer and superoxide production in bystander cancer cells. Cells, 8(10), 1175.

Scholl, B., Wilson, D. E., Jaepel, J., & Fitzpatrick, D. (2019). Functional logic of layer 2/3 inhibitory connectivity in the ferret visual cortex. Neuron, 104(3), 451-457.

Ceriani, F., Johnson, S. L., Sedlacek, M., Hendry, A., Kachar, B., Marcotti, W., & Mammano, F. (2019). Dynamic coupling of cochlear inner hair cell intrinsic Ca²⁺ action potentials to Ca²⁺ signaling of non-sensory cells. bioRxiv, 731851.

Brawek, B and Garaschuk O. (2019). Single-cell electroporation for measuring in vivo calcium dynamics in microglia. Microglia Methods and Protocols, 231-241.

Brawek, B., Olmedillas del Moral, M., & Garaschuk, O. (2019). In vivo visualization of microglia using tomato lectin. Microglia Methods and Protocols, 165-175.

Liang, Y. & Garaschuk O. (2019). Labeling microglia with genetically encoded calcium indicators. Microglia Methods and Protocols, 243–265.

Royzen, F., Williams, S., Fernandez, F. R., & White, J. A. (2019). Balanced synaptic currents underlie low-frequency oscillations in the subiculum. Hippocampus, 29(12), 1178-1189.

Rathore, A. P. S., Mantri, C. K., Aman, S. A. B., Syenina, A., Ooi, J., Jagaraj, C. J., ... & St. John, A. L. (2019). Dengue virus-elicited tryptase induces endothelial permeability and shock. Journal of Clinical Investiagtion, 129(10), 4180-4193.

Stringer, C., Pachitariu, M., Steinmetz, N., Carandini, M., & Harris, K. D. (2019). High-dimensional geometry of population responses in visual cortex. Nature, 571(7765), 361–365.

Ziraldo, G., Buratto, D., Kuang, Y., Xu, L., Carrer, A., Nardin, C., ... & Mammano, F. (2019). A human-derived monoclonal antibody targeting extracellular connexin domain selectively modulates hemichannel function. Frontiers in Physiology, 10, 392.

Díaz-García, C. M., Lahmann, C., Martínez-François, J. R., Li, B., Koveal, D., Nathwani N., ... & Yellen, G. (2019). Quantitative in vivo imaging of neuronal glucose concentrations with a genetically encoded fluorescence lifetime sensor. Journal of Neuroscience Research, 97(8), 946–960.

Philip, V., Newton, D., Oh, H., Collins, S., Bercik, P., & Sibille, E. (2019). The Effect of Gut Microbiota on Glutamatergic/GABAergic Gene Expression in Adult Mice. Biological Psychiatry, 85, S127–S128.

Bowen, Z., Winkowski, D. E., Seshadri, S., Plenz, D., & Kanold, P. O. (2019). Neuronal avalanches in input and associative layers of auditory cortex. Frontiers in Systems Neuroscience, 13, 45.

Burgold, J., Schulz-Trieglaff, E. K., Voelkl, K., Gutiérrez-Ángel, S., Bader, J. M., Hosp, F., ... & Dudanova, I. (2019). Cortical circuit alterations precede motor impairments in Huntington’s disease mice. Scientific Reports, 9(1), 6634.

Matovic, S., Ichiyama, A., Igarashi, H., Salter, E. W., Wang, X. F., Henry, M., ... & Inoue, W. (2019). Stress-induced neuronal hypertrophy decreases the intrinsic excitability in stress habituation. bioRxiv, 593665.

Weissenberger, Y., King, A. J., & Dahmen, J. C. (2019). Decoding mouse behavior to explain single-trial decisions and their relationship with neural activity. bioRxiv, 567479.

Ceriani, F., Hendry, A., Jeng, J. Y., Johnson, S. L., Stephani, F., Olt, J., ... & Marcotti, W. (2019). Coordinated calcium signalling in cochlear sensory and non-sensory cells refines afferent innervation of outer hair cells. The EMBO Journal, 38(9), e99839.

Lee, K. S., Vandemark, K., Mezey, D., Shultz, N., & Fitzpatrick, D. (2019). Functional synaptic architecture of callosal inputs in mouse primary visual cortex. Neuron, 101(3), 421-428.

2018

Marvin, J. S., Scholl, B., Wilson, D. E., Podgorski, K., Kazemipour, A., Müller, J. A., ... & Looger, L. L. (2018). Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nature Methods, 15(11), 936–939.

Moeyaert, B., Holt, G., Madangopal, R., Perez-Alvarez, A., Fearey, B. C., Trojanowski, N. F., ... & Schreiter, E. R. (2018). Improved methods for marking active neuron populations. Nature Communications, 9(1), 440.

Chen, C. L., Hermans, L., Viswanathan, M. C., Fortun, D., Aymanns, F., Unser, M., ... & Ramdya, P. (2018). Imaging neural activity in the ventral nerve cord of behaving adult Drosophila. Nature Communications, 9(1), 4390.

Corns, L. F., Johnson, S. L., Roberts, T., Ranatunga, K. M., Hendry, A., Ceriani, F., ... & Marcotti, W. (2018). Mechanotransduction is required for establishing and maintaining mature inner hair cells and regulating efferent innervation. Nature Communications, 9(1), 4015.

Saleem, A. B., Diamanti, E. M., Fournier, J., Harris, K. D., & Carandini, M. (2018). Coherent encoding of subjective spatial position in visual cortex and hippocampus. Nature, 562(7725), 124-127.

Dipoppa, M., Ranson, A., Krumin, M., Pachitariu, M., Carandini, M., & Harris, K. D. (2018). Vision and locomotion shape the interactions between neuron types in mouse visual cortex. Neuron, 98(3), 602-615.

Gillet, S. N., Kato, H. K., Justen, M. A., Lai, M., & Isaacson, J. S. (2018). Fear learning regulates cortical sensory representations by suppressing habituation. Frontiers in Neural Circuits, 11, 112.

2017

Scholl, B., Wilson, D. E., & Fitzpatrick, D. (2017). Local order within global disorder: synaptic architecture of visual space. Neuron, 96(5), 1127-1138.

Klapoetke, N. C., Nern, A., Peek, M. Y., Rogers, E. M., Breads, P., Rubin, G. M., ... & Card, G. M. (2017). Ultra-selective looming detection from radial motion opponency. Nature Neuroscience, 551(7679), 237-241.

Kato, H. K., Asinof, S. K., & Isaacson, J. S. (2017). Network-level control of frequency tuning in auditory cortex. Neuron, 95(2), 412-423.

Lu, R., Sun, W., Liang, Y., Kerlin, A., Bierfeld, J., Seelig, J.D., W... & Ji, N. (2017). Video-rate volumetric functional imaging of the brain at synaptic resolution. Nature Neuroscience, 20(4), 620-628.

2016

Mongeon, R., Venkatachalam, V., & Yellen, G. (2016). Cytosolic NADH-NAD+ redox visualized in brain slices by two-photon fluorescence lifetime biosensor imaging.. Antioxid Redox Signal, 25(10), 553-563.

Pachitariu, M., Stringer, C., Schröder, S., Dipoppa, M., Rossi, L. F., Carandini, M., & Harris, K. D. (2016). Suite2p: beyond 10,000 neurons with standard two-photon microscopy. bioRxiv, 061507.

Rose, T., Jaepel, J., Hübener, M., & Bonhoeffer, T. (2016). Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex. Science, 352(6291), 1319–1322.

Strobl, M. J., Freeman, D., Patel, J., Poulsen, R., Wendler, C. C., Rivkees, S. A., & Coleman, J. E. (2016). Opposing effects of maternal hypo-and hyperthyroidism on the stability of thalamocortical synapses in the visual cortex of adult offspring.. Cerebral Cortex, 27(5), 3015-3027.

Lee, K. S., Huang, X., & Fitzpatrick, D. (2016). Topology of ON and OFF inputs in visual cortex enables an invariant columnar architecture. Nature, 533(7601), 90-94.

Monai, H., Ohkura, M., Tanaka, M., Oe, Y., Konno, A., Hirai, H., ... & Hirase, H. (2016). Calcium imaginq reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nature Communications, 7(1), 11100.

Ganmor, E., Krumin, M., Rossi, L. F., Carandini, M., & Simoncelli, E. P. (2016). Direct estimation of firing rates from calcium imaging data. arXiv, 1601.00364.

2015

Roth, M. M., Dahmen, J.C., Muir, D. R., Imhof, F., Martini, F. J., & Hofer, S. B. (2015). Thalamic nuclei convey diverse contextual information to layer 1 of visual cortex. Nature Neuroscience, 19(2), 299-307.

Barnstedt, O., Keating, P., Weissenberger, Y., King, A. J., & Dahmen, J. C. (2015). Functional microarchitecture of the mouse dorsal inferior colliculus revealed through in vivo two-photon calcium imaging.. Journal of Neuroscience, 35(31), 10927-10939.

Chen, S. X., Kim, A. N., Peters, A. J., & Komiyama, T. (2015). Subtype-specific plasticity of inhibitory circuits in motor cortex during motor learning. Nature Neuroscience, 18(8), 1109-1115.

Jia, Y., Zhang, S., Miao, L., Wang, J., Jin, Z., Gu, B., ... & Li, Z. (2015). Activation of platelet protease-activated receptor-1 induces epithelial-mesenchymal transition and chemotaxis of colon cancer cell line SW620. Oncology Reports, 33(6), 2681-2688.

Lu, W., Tang, Y., Zhang, Z., Zhang, X., Yao, Y., Fu, C., ... & Ma, G. (2015). Inhibiting the mobilization of Ly6Chigh monocytes after acute myocardial infarction enhances the efficiency of mesenchymal stromal cell transplantation and curbs myocardial remodeling.. American Journal of Translational Research, 7(3), 587-597.

Boyd, A. M., Kato, H. K., Komiyama, T., & Isaacson, J. S. (2015). Broadcasting of cortical activity to the olfactory bulb. Cell Reports, 10(7), 1032-1039.

Cossell, L., Iacaruso, M. F., Muir, D. R., Houlton, R., Sader, E. N., Ko, H., H... & Mrsic-Flogel, T. D. (2015). Functional organization of excitatory synaptic strength in primary visual cortex. Nature, 518(7539), 399-403.

2014

Partridge, J. G., Lewin, A. E., Yasko, J. R., & Vicini, S. (2014). Contrasting actions of group I metabotropic glutamate receptors in distinct mouse striatal neurones. Journal of Physiology, 592(13), 2721-2733.

Peters, A. J., Chen, S, X., Komiyama, T. (2014). Emergence of reproducible spatiotemporal activity during motor learning. Nature, 510(7504), 263-267.

Ehmke, T., Nitzsche, T. H., Knebl, A., & Heisterkamp, A. (2014). Molecular orientation sensitive second harmonic microscopy by radially and azimuthally polarized light. Biomedical Optics Express, 5(7), 2231-46.

Liu, J., Wu, N., Ma, L., Liu, M., Liu, G., Zhang, Y., & Lin, X. (2014). Oleanolic acid suppresses aerobic glycolysis in cancer cells by switching pyruvate kinase type M isoforms. PLoS One, 9(3), e91606.

Palmer, L. M., Shai, A. S., Reeve, J.E., Anderson, H. L., Paulsen, O., & Larkum, M. E. (2014). NMDA spikes enhance action potential generation during sensory input. Nature Neuroscience, 17(3), 383-390.

Cai, F., Yu, J., Qian, J., Wang, Y., Chen, Z., Huang, J., ... & He, S. (2014). Use of tunable second-harmonic signal from KNbO₃ nanoneedles to find optimal wavelength for deep-tissue imaging. Laser & Photonics Reviews, 8(6), 865-874.

2013

Kato, H. K., Gillet, S. N., Peters, A. J., Isaacson, J. S., & Komiyama, T. (2013). Parvalbumin-expressing interneurons linearly control olfactory bulb output. Neuron, 80(5), 1218-1231.

Takata, N., Nagai, T., Ozawa, K., Oe, Y., Mikoshiba, K., & Hirase, H. (2013). Cerebral blood flow modulation by Basal forebrain or whisker stimulation can occur independently of large cytosolic Ca²⁺ signaling in astrocytes. PLoS One, 8(6), e66525.

製品パンフレットとマインドマップ

Bergamo^® IIシリーズ顕微鏡の各資料(pdf)は下のボタンをクリックするとご覧いただけます。